Original Article

The effect of Wharton's jelly mesenchymal stem cells in colorectal cancer

Fatemeh Mollaei , Mohammad Ali

Fatemeh Mollaei
Medical Genetics Research Center, Faculty of Medical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran. Email: fatima.mollaie@gmail.com

Mohammad Ali
Institute of Molecular biology and Biotechnology, University of Lahore, Lahore, Pakistan
Online First: September 26, 2017 | Cite this Article
Mollaei, F., Ali, M. 2017. The effect of Wharton's jelly mesenchymal stem cells in colorectal cancer. The Cancer Press 3(3): 86-102. DOI:10.15562/tcp.48

Mesenchymal Stem Cells (MSCs) are self-renewing multipotent cells that can be isolated from various organs including Wharton’s jelly of umbilical cord (WJMSCs). WJMSCs play a fundamental role in field of regenerative medicine and hold therapeutic potential for various diseases including gene therapy of different cancers. WJMSCs were evaluated for their potential behavior in cancer conditions by culturing them in the plasma obtained from colorectal cancer patients. The cancer plasma was tested LDH release, paracrine factors (p53, p38, BAX and BCl2) and oxidative stress. Cell viability, death and proliferation was assessed by crystal violet staining, LDH release and MTT assay respectively. Assessment of cancer plasma expressed high LDH release, low expression of pro-apoptotic gene BAX, high expression of survival gene BCl2 p38 kinase and tumor suppressor gene p53. In oxidative stress, GSH and SOD were increased in CRC. In CAT, it was highly expressed in CRC. Furthermore, increased level of MDA was expressed in cancers’ plasma. It was observed that cells cultured in plasma of cancer demonstrate less viability, low proliferation rate, high LDH release, low expression of BAX and p38 kinase, whereas, high expression of BCL2 and p53. In oxidative stress, low GSH, SOD, CAT and high MDA was observed in CRC. When WJMSCs cultured in plasma from colorectal cancer patients these cells show reduced viability, proliferation and increased apoptosis. For the future, certain measures should be taken to improve viability of WJMSCs in cancer conditions.  

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